Journal: Journal of Immunology Research

Article Title: Efficacy of Primate Humoral Passive Transfer in a Murine Model of Pneumonic Plague Is Mouse Strain-Dependent

doi: 10.1155/2014/807564

Figure Lengend Snippet: Pharmacokinetics of human or cynomolgus macaque antibody in BALB/c and Hsd:NIHS mice, respectively. 0.25 mL human ((a) and (c)) or cynomolgus macaque ((b) and (d)) plague vaccine serum was injected via the intraperitoneal route into BALB/c ( n = 20) or Hsd:NIHS ( n = 20) mice, respectively. The mice were serially sacrificed and the presence of anti-rF1 ((a) and (b)) or anti-rV ((c) and (d)) antibody was assessed by ELISA. Additional groups of mice ( n = 5) were sacrificed preinjection to provide baseline data. The data suggests that the time delay between passive antibody administration and optimal serum concentration in the murine serum was between 3 and 6 hours. a.u., arbitrary units.

Article Snippet: Two cynomolgus macaques were immunized then twice boosted with a recombinant plague vaccine consisting of rF1 and rV antigens with an alhydrogel adjuvant (plague (recombinant) vaccine suspension, 873082-H01, Batch 7051, Avecia Biologics Ltd., UK).

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: Journal of Immunology Research

Article Title: Efficacy of Primate Humoral Passive Transfer in a Murine Model of Pneumonic Plague Is Mouse Strain-Dependent

doi: 10.1155/2014/807564

Figure Lengend Snippet: When all of the passive protection data from BALB/c mice were pooled, the log ELISA titre against both rF1 and rV correlated with survival time.

Article Snippet: Two cynomolgus macaques were immunized then twice boosted with a recombinant plague vaccine consisting of rF1 and rV antigens with an alhydrogel adjuvant (plague (recombinant) vaccine suspension, 873082-H01, Batch 7051, Avecia Biologics Ltd., UK).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of Immunology Research

Article Title: Efficacy of Primate Humoral Passive Transfer in a Murine Model of Pneumonic Plague Is Mouse Strain-Dependent

doi: 10.1155/2014/807564

Figure Lengend Snippet: Assessment of anti-rF1 and anti-rV antibody titres in NHP serum after 3 intramuscular immunizations of 10 μ g of rF1 and rV antigen. Detectable amounts of anti-rF1 and anti-rV antibodies were found in the serum 2 weeks after the primary immunization, with anti-rF1 consistently having a higher titre.

Article Snippet: Two cynomolgus macaques were immunized then twice boosted with a recombinant plague vaccine consisting of rF1 and rV antigens with an alhydrogel adjuvant (plague (recombinant) vaccine suspension, 873082-H01, Batch 7051, Avecia Biologics Ltd., UK).

Techniques:

Journal: Vaccine

Article Title: UK vaccines network: Mapping priority pathogens of epidemic potential and vaccine pipeline developments

doi: 10.1016/j.vaccine.2019.09.009

Figure Lengend Snippet: Vaccines reported as tested clinically against UKVN priority diseases.* where peer reviewed results are available the reference has been included, otherwise the ClinicalTrials.gov identifier has been listed. rV = Recombinant virus vaccine, iV = Inactivated virus vaccine, iB = inactivated Bacterial vaccine, VLP = Virus like particle vaccine, Sub = Subunit vaccine, N = Nanoparticle, IgG = Therapeutic antibody, DNA = DNA vaccine, VSV = Vesicular stomatitis virus, Ad5, Ad26 = replication defective Human Adenovirus type 5 and type 26 respectively, ChAd1 = Chimpanzee Adenovirus type 1, ChAd3 = Chimpanzee Adenovirus type 3, MVA = Modified Vaccinia virus Ankara, CHIKV = Chikungunya virus, CCHF = Crimean-Congo Haemorrhagic Fever virus, RVFV = Rift Valley Fever Virus, SARS = Severe Acute Respiratory Syndrome, MERS = Middle East Respiratory Syndrome.

Article Snippet: Plague , rF1V vaccine , Sub , – , II , DynPort Vaccine Company LLC , NCT00332956.

Techniques: Vaccines, Recombinant, Virus, Modification, Plasmid Preparation